Review



r mucilaginosa  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 95
      Buy from Supplier

    Structured Review

    ATCC r mucilaginosa
    Physical sectioning improves hybridization of Gram-positive bacteria. Bacterial cells were hybridized under identical conditions either as whole cell mounts (left) or after embedding and sectioning (right) and imaged with confocal microscopy. Differential interference contrast (DIC) microscopy images are shown to display all cells in the field of view. Fluorescence images display hybridization signal from the FISH probe Eub338-Dy505. Across individual species, image acquisition and display settings were kept constant to allow comparison between the two sample preparations. In whole mounts, only a small fraction of the cells stain brightly. In sections, fluorescence signal is homogeneous, and most cells stain brightly. R. <t>mucilaginosa</t> , which hybridizes well in whole cell mounts, is shown as a positive control. Scale bar = 10 microns.
    R Mucilaginosa, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/r mucilaginosa/product/ATCC
    Average 95 stars, based on 47 article reviews
    r mucilaginosa - by Bioz Stars, 2026-02
    95/100 stars

    Images

    1) Product Images from "Slicing overcomes the bacterial cell wall barrier to fluorescence in situ hybridization"

    Article Title: Slicing overcomes the bacterial cell wall barrier to fluorescence in situ hybridization

    Journal: Microbiology Spectrum

    doi: 10.1128/spectrum.02001-25

    Physical sectioning improves hybridization of Gram-positive bacteria. Bacterial cells were hybridized under identical conditions either as whole cell mounts (left) or after embedding and sectioning (right) and imaged with confocal microscopy. Differential interference contrast (DIC) microscopy images are shown to display all cells in the field of view. Fluorescence images display hybridization signal from the FISH probe Eub338-Dy505. Across individual species, image acquisition and display settings were kept constant to allow comparison between the two sample preparations. In whole mounts, only a small fraction of the cells stain brightly. In sections, fluorescence signal is homogeneous, and most cells stain brightly. R. mucilaginosa , which hybridizes well in whole cell mounts, is shown as a positive control. Scale bar = 10 microns.
    Figure Legend Snippet: Physical sectioning improves hybridization of Gram-positive bacteria. Bacterial cells were hybridized under identical conditions either as whole cell mounts (left) or after embedding and sectioning (right) and imaged with confocal microscopy. Differential interference contrast (DIC) microscopy images are shown to display all cells in the field of view. Fluorescence images display hybridization signal from the FISH probe Eub338-Dy505. Across individual species, image acquisition and display settings were kept constant to allow comparison between the two sample preparations. In whole mounts, only a small fraction of the cells stain brightly. In sections, fluorescence signal is homogeneous, and most cells stain brightly. R. mucilaginosa , which hybridizes well in whole cell mounts, is shown as a positive control. Scale bar = 10 microns.

    Techniques Used: Hybridization, Bacteria, Confocal Microscopy, Microscopy, Fluorescence, Comparison, Staining, Positive Control

    Hybridization in sections depends on bacterial size and morphology. Confocal Z-stacks were acquired from 5 μm thick sections of three difficult-to-hybridize Gram-positive taxa, Streptococcus salivarius (Str. sal .), Schaalia odontolytica (Sch. odo .), and Corynebacterium matruchotii (Cor. mat .), differing in size and shape. A fourth taxon, Rothia mucilaginosa (Rot. muc .), that hybridizes readily in whole cell mounts was included as a positive control. ( A ) Fluorescence image of the center focal plane of the physical section. S. salivarius and S. odontolytica show non-homogeneous hybridization in this plane. ( B ) Orthogonal view of the YZ-plane indicated by the yellow line in ( A ) showing a strong signal at the cut faces and a dramatic reduction of signal in the interior of the section for taxa with cocci or rod morphology, whereas the filamentous taxon, C. matruchotii , does not show this reduction. ( C ) Average intensity projection across all YZ planes. ( D ) Enlarged view of the yellow box shown in panel C . Hybridization extended further into the section for filamentous C. matruchotii . ( E ) Plots displaying average image intensity across all Z-positions in microns, where zero represents the center focal plane of the physical section. The morphology of the bacterium correlates with the level of hybridization in the interior of the section. White scale bar = 10 microns. Red scale bar = 5 microns. The image shown in the first row of column A is the same as the image labeled “0.00” in .
    Figure Legend Snippet: Hybridization in sections depends on bacterial size and morphology. Confocal Z-stacks were acquired from 5 μm thick sections of three difficult-to-hybridize Gram-positive taxa, Streptococcus salivarius (Str. sal .), Schaalia odontolytica (Sch. odo .), and Corynebacterium matruchotii (Cor. mat .), differing in size and shape. A fourth taxon, Rothia mucilaginosa (Rot. muc .), that hybridizes readily in whole cell mounts was included as a positive control. ( A ) Fluorescence image of the center focal plane of the physical section. S. salivarius and S. odontolytica show non-homogeneous hybridization in this plane. ( B ) Orthogonal view of the YZ-plane indicated by the yellow line in ( A ) showing a strong signal at the cut faces and a dramatic reduction of signal in the interior of the section for taxa with cocci or rod morphology, whereas the filamentous taxon, C. matruchotii , does not show this reduction. ( C ) Average intensity projection across all YZ planes. ( D ) Enlarged view of the yellow box shown in panel C . Hybridization extended further into the section for filamentous C. matruchotii . ( E ) Plots displaying average image intensity across all Z-positions in microns, where zero represents the center focal plane of the physical section. The morphology of the bacterium correlates with the level of hybridization in the interior of the section. White scale bar = 10 microns. Red scale bar = 5 microns. The image shown in the first row of column A is the same as the image labeled “0.00” in .

    Techniques Used: Hybridization, Positive Control, Fluorescence, Labeling



    Similar Products

    r mucilaginosa  (ATCC)
    95
    ATCC r mucilaginosa
    Physical sectioning improves hybridization of Gram-positive bacteria. Bacterial cells were hybridized under identical conditions either as whole cell mounts (left) or after embedding and sectioning (right) and imaged with confocal microscopy. Differential interference contrast (DIC) microscopy images are shown to display all cells in the field of view. Fluorescence images display hybridization signal from the FISH probe Eub338-Dy505. Across individual species, image acquisition and display settings were kept constant to allow comparison between the two sample preparations. In whole mounts, only a small fraction of the cells stain brightly. In sections, fluorescence signal is homogeneous, and most cells stain brightly. R. <t>mucilaginosa</t> , which hybridizes well in whole cell mounts, is shown as a positive control. Scale bar = 10 microns.
    R Mucilaginosa, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/r mucilaginosa/product/ATCC
    Average 95 stars, based on 1 article reviews
    r mucilaginosa - by Bioz Stars, 2026-02
    95/100 stars
    		  Buy from Supplier
    r mucilaginosa atcc 25296  (ATCC)
    95
    ATCC r mucilaginosa atcc 25296
    Physical sectioning improves hybridization of Gram-positive bacteria. Bacterial cells were hybridized under identical conditions either as whole cell mounts (left) or after embedding and sectioning (right) and imaged with confocal microscopy. Differential interference contrast (DIC) microscopy images are shown to display all cells in the field of view. Fluorescence images display hybridization signal from the FISH probe Eub338-Dy505. Across individual species, image acquisition and display settings were kept constant to allow comparison between the two sample preparations. In whole mounts, only a small fraction of the cells stain brightly. In sections, fluorescence signal is homogeneous, and most cells stain brightly. R. <t>mucilaginosa</t> , which hybridizes well in whole cell mounts, is shown as a positive control. Scale bar = 10 microns.
    R Mucilaginosa Atcc 25296, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/r mucilaginosa atcc 25296/product/ATCC
    Average 95 stars, based on 1 article reviews
    r mucilaginosa atcc 25296 - by Bioz Stars, 2026-02
    95/100 stars
    		  Buy from Supplier
    r. mucilaginosa strain  (China Center for Type Culture Collection)
    90
    China Center for Type Culture Collection r. mucilaginosa strain
    Physical sectioning improves hybridization of Gram-positive bacteria. Bacterial cells were hybridized under identical conditions either as whole cell mounts (left) or after embedding and sectioning (right) and imaged with confocal microscopy. Differential interference contrast (DIC) microscopy images are shown to display all cells in the field of view. Fluorescence images display hybridization signal from the FISH probe Eub338-Dy505. Across individual species, image acquisition and display settings were kept constant to allow comparison between the two sample preparations. In whole mounts, only a small fraction of the cells stain brightly. In sections, fluorescence signal is homogeneous, and most cells stain brightly. R. <t>mucilaginosa</t> , which hybridizes well in whole cell mounts, is shown as a positive control. Scale bar = 10 microns.
    R. Mucilaginosa Strain, supplied by China Center for Type Culture Collection, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/r. mucilaginosa strain/product/China Center for Type Culture Collection
    Average 90 stars, based on 1 article reviews
    r. mucilaginosa strain - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier
    r mucilaginosa strains atcc 58901  (ATCC)
    94
    ATCC r mucilaginosa strains atcc 58901
    Physical sectioning improves hybridization of Gram-positive bacteria. Bacterial cells were hybridized under identical conditions either as whole cell mounts (left) or after embedding and sectioning (right) and imaged with confocal microscopy. Differential interference contrast (DIC) microscopy images are shown to display all cells in the field of view. Fluorescence images display hybridization signal from the FISH probe Eub338-Dy505. Across individual species, image acquisition and display settings were kept constant to allow comparison between the two sample preparations. In whole mounts, only a small fraction of the cells stain brightly. In sections, fluorescence signal is homogeneous, and most cells stain brightly. R. <t>mucilaginosa</t> , which hybridizes well in whole cell mounts, is shown as a positive control. Scale bar = 10 microns.
    R Mucilaginosa Strains Atcc 58901, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/r mucilaginosa strains atcc 58901/product/ATCC
    Average 94 stars, based on 1 article reviews
    r mucilaginosa strains atcc 58901 - by Bioz Stars, 2026-02
    94/100 stars
    		  Buy from Supplier
    annotated r mucilaginosa genomes  (ATCC)
    94
    ATCC annotated r mucilaginosa genomes
    Taxonomic identification of yeasts isolated from larvae and water samples collected from Ae. albopictus breeding sites. Field sampling was performed in September–October 2019 within a community garden localized in Saint Priest (Metropolis of Lyon, France). The 10 waterborne yeasts colonizing the gut of Ae. albopictus larvae were selected to evaluate their capacity to secrete uricase and impact mosquito development
    Annotated R Mucilaginosa Genomes, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/annotated r mucilaginosa genomes/product/ATCC
    Average 94 stars, based on 1 article reviews
    annotated r mucilaginosa genomes - by Bioz Stars, 2026-02
    94/100 stars
    		  Buy from Supplier
    r mucilaginosa  (DSMZ)
    94
    DSMZ r mucilaginosa
    Fig. 1 | Early-life fungal colonization and diet-induced obesity model. A Gnotobiotic colonization and diet-induced obesity model. Germ-free dams were gavaged twice with the Oligo-MM12 consortium alone (B; gray) or in combination with C. albicans (B + C; orange), R. <t>mucilaginosa</t> (B + R; pink) or M. restricta (B + M; purple; Males—SD: nB = 23, nB+C = 8, nB+R = 10, nB+M = 12; Females—SD: nB = 18, nB+C = 8, nB+R = 8, nB+M = 12; Males—HFHS: nB = 22, nB+C = 10, nB+R = 8, nB+M = 12; Females—HFHS: nB = 20, nB+C = 12, nB+R = 7, nB+M = 10). Twice in the first week of life, offspring (F1) were exposed to the fungal species corresponding to their coloniza- tion group. F1 were weaned onto standard diet (SD) or high-fat-high-sucrose diet
    R Mucilaginosa, supplied by DSMZ, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/r mucilaginosa/product/DSMZ
    Average 94 stars, based on 1 article reviews
    r mucilaginosa - by Bioz Stars, 2026-02
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    Physical sectioning improves hybridization of Gram-positive bacteria. Bacterial cells were hybridized under identical conditions either as whole cell mounts (left) or after embedding and sectioning (right) and imaged with confocal microscopy. Differential interference contrast (DIC) microscopy images are shown to display all cells in the field of view. Fluorescence images display hybridization signal from the FISH probe Eub338-Dy505. Across individual species, image acquisition and display settings were kept constant to allow comparison between the two sample preparations. In whole mounts, only a small fraction of the cells stain brightly. In sections, fluorescence signal is homogeneous, and most cells stain brightly. R. mucilaginosa , which hybridizes well in whole cell mounts, is shown as a positive control. Scale bar = 10 microns.

    Journal: Microbiology Spectrum

    Article Title: Slicing overcomes the bacterial cell wall barrier to fluorescence in situ hybridization

    doi: 10.1128/spectrum.02001-25

    Figure Lengend Snippet: Physical sectioning improves hybridization of Gram-positive bacteria. Bacterial cells were hybridized under identical conditions either as whole cell mounts (left) or after embedding and sectioning (right) and imaged with confocal microscopy. Differential interference contrast (DIC) microscopy images are shown to display all cells in the field of view. Fluorescence images display hybridization signal from the FISH probe Eub338-Dy505. Across individual species, image acquisition and display settings were kept constant to allow comparison between the two sample preparations. In whole mounts, only a small fraction of the cells stain brightly. In sections, fluorescence signal is homogeneous, and most cells stain brightly. R. mucilaginosa , which hybridizes well in whole cell mounts, is shown as a positive control. Scale bar = 10 microns.

    Article Snippet: S. salivarius (ATCC 7073), R. mucilaginosa (ATCC 25296), and C. matruchotii (ATCC 14266) were grown under aerobic conditions.

    Techniques: Hybridization, Bacteria, Confocal Microscopy, Microscopy, Fluorescence, Comparison, Staining, Positive Control

    Hybridization in sections depends on bacterial size and morphology. Confocal Z-stacks were acquired from 5 μm thick sections of three difficult-to-hybridize Gram-positive taxa, Streptococcus salivarius (Str. sal .), Schaalia odontolytica (Sch. odo .), and Corynebacterium matruchotii (Cor. mat .), differing in size and shape. A fourth taxon, Rothia mucilaginosa (Rot. muc .), that hybridizes readily in whole cell mounts was included as a positive control. ( A ) Fluorescence image of the center focal plane of the physical section. S. salivarius and S. odontolytica show non-homogeneous hybridization in this plane. ( B ) Orthogonal view of the YZ-plane indicated by the yellow line in ( A ) showing a strong signal at the cut faces and a dramatic reduction of signal in the interior of the section for taxa with cocci or rod morphology, whereas the filamentous taxon, C. matruchotii , does not show this reduction. ( C ) Average intensity projection across all YZ planes. ( D ) Enlarged view of the yellow box shown in panel C . Hybridization extended further into the section for filamentous C. matruchotii . ( E ) Plots displaying average image intensity across all Z-positions in microns, where zero represents the center focal plane of the physical section. The morphology of the bacterium correlates with the level of hybridization in the interior of the section. White scale bar = 10 microns. Red scale bar = 5 microns. The image shown in the first row of column A is the same as the image labeled “0.00” in .

    Journal: Microbiology Spectrum

    Article Title: Slicing overcomes the bacterial cell wall barrier to fluorescence in situ hybridization

    doi: 10.1128/spectrum.02001-25

    Figure Lengend Snippet: Hybridization in sections depends on bacterial size and morphology. Confocal Z-stacks were acquired from 5 μm thick sections of three difficult-to-hybridize Gram-positive taxa, Streptococcus salivarius (Str. sal .), Schaalia odontolytica (Sch. odo .), and Corynebacterium matruchotii (Cor. mat .), differing in size and shape. A fourth taxon, Rothia mucilaginosa (Rot. muc .), that hybridizes readily in whole cell mounts was included as a positive control. ( A ) Fluorescence image of the center focal plane of the physical section. S. salivarius and S. odontolytica show non-homogeneous hybridization in this plane. ( B ) Orthogonal view of the YZ-plane indicated by the yellow line in ( A ) showing a strong signal at the cut faces and a dramatic reduction of signal in the interior of the section for taxa with cocci or rod morphology, whereas the filamentous taxon, C. matruchotii , does not show this reduction. ( C ) Average intensity projection across all YZ planes. ( D ) Enlarged view of the yellow box shown in panel C . Hybridization extended further into the section for filamentous C. matruchotii . ( E ) Plots displaying average image intensity across all Z-positions in microns, where zero represents the center focal plane of the physical section. The morphology of the bacterium correlates with the level of hybridization in the interior of the section. White scale bar = 10 microns. Red scale bar = 5 microns. The image shown in the first row of column A is the same as the image labeled “0.00” in .

    Article Snippet: S. salivarius (ATCC 7073), R. mucilaginosa (ATCC 25296), and C. matruchotii (ATCC 14266) were grown under aerobic conditions.

    Techniques: Hybridization, Positive Control, Fluorescence, Labeling

    Taxonomic identification of yeasts isolated from larvae and water samples collected from Ae. albopictus breeding sites. Field sampling was performed in September–October 2019 within a community garden localized in Saint Priest (Metropolis of Lyon, France). The 10 waterborne yeasts colonizing the gut of Ae. albopictus larvae were selected to evaluate their capacity to secrete uricase and impact mosquito development

    Journal: Microbiome

    Article Title: Environmental yeasts differentially impact the development and oviposition behavior of the Asian tiger mosquito Aedes albopictus

    doi: 10.1186/s40168-025-02099-6

    Figure Lengend Snippet: Taxonomic identification of yeasts isolated from larvae and water samples collected from Ae. albopictus breeding sites. Field sampling was performed in September–October 2019 within a community garden localized in Saint Priest (Metropolis of Lyon, France). The 10 waterborne yeasts colonizing the gut of Ae. albopictus larvae were selected to evaluate their capacity to secrete uricase and impact mosquito development

    Article Snippet: An analysis of all publicly available, assembled, and annotated R. mucilaginosa genomes (strains ATCC 58901 and J31 from the JGI MycoCosm portal, as well as the reference strain GDMCC2.30 from NCBI GenBank, accession number PRJNA1034680) confirmed that R. mucilaginosa possess the complete set of genes encoding the uricolytic/ureolytic enzymes required for amino acids synthesis through nitrogen waste recycling.

    Techniques: Isolation, Sampling

    Uric acid recycling pathway present in R. mucilaginosa strains, including the one isolated from both Ae. albopictus larvae and water-breeding sites. An analysis of all publicly available, assembled, and annotated R. mucilaginosa genomes (strains ATCC 58901 and J31 from the JGI MycoCosm portal, as well as the reference strain GDMCC2.30 from NCBI GenBank, accession number PRJNA1034680) confirmed that R. mucilaginosa possess the complete set of genes encoding the uricolytic/ureolytic enzymes required for amino acids synthesis through nitrogen waste recycling. Some of these genes (blue arrows) were identified as expressed in an available transcriptome , and their presence in the genome of the strain used in this study was confirmed by PCR (red stars)

    Journal: Microbiome

    Article Title: Environmental yeasts differentially impact the development and oviposition behavior of the Asian tiger mosquito Aedes albopictus

    doi: 10.1186/s40168-025-02099-6

    Figure Lengend Snippet: Uric acid recycling pathway present in R. mucilaginosa strains, including the one isolated from both Ae. albopictus larvae and water-breeding sites. An analysis of all publicly available, assembled, and annotated R. mucilaginosa genomes (strains ATCC 58901 and J31 from the JGI MycoCosm portal, as well as the reference strain GDMCC2.30 from NCBI GenBank, accession number PRJNA1034680) confirmed that R. mucilaginosa possess the complete set of genes encoding the uricolytic/ureolytic enzymes required for amino acids synthesis through nitrogen waste recycling. Some of these genes (blue arrows) were identified as expressed in an available transcriptome , and their presence in the genome of the strain used in this study was confirmed by PCR (red stars)

    Article Snippet: An analysis of all publicly available, assembled, and annotated R. mucilaginosa genomes (strains ATCC 58901 and J31 from the JGI MycoCosm portal, as well as the reference strain GDMCC2.30 from NCBI GenBank, accession number PRJNA1034680) confirmed that R. mucilaginosa possess the complete set of genes encoding the uricolytic/ureolytic enzymes required for amino acids synthesis through nitrogen waste recycling.

    Techniques: Isolation

    Impact of acetohydroxamic acid-mediated urease inhibition on the development time of gnotobiotic Ae. albopictus larvae associated with uricolytic/ureolytic ( R. mucilaginosa ) and non-uricolytic/ureolytic ( M. asiatica ) yeast species. To confirm the involvement of the yeast uric acid recycling pathway in larval development, we inhibited it by targeting urease, the only enzyme in the yeast recycling pathway absent from the Asian tiger mosquito genome. The impact of this inhibition on the development time of gnotobiotic mosquitoes ( n = 60 per infection status) was assessed using an inhibitor concentration of 2.5 mM. Prior to this experiment, we confirmed that this inhibitor concentration effectively inhibited R. mucilaginosa urease activity without affecting yeast growth or axenic larval development. Statistically significant differences between groups were identified with the Tukey post hoc tests. Columns labelled with an asterisk (*) are significantly different with a p -value < 0.05. ns, not significant

    Journal: Microbiome

    Article Title: Environmental yeasts differentially impact the development and oviposition behavior of the Asian tiger mosquito Aedes albopictus

    doi: 10.1186/s40168-025-02099-6

    Figure Lengend Snippet: Impact of acetohydroxamic acid-mediated urease inhibition on the development time of gnotobiotic Ae. albopictus larvae associated with uricolytic/ureolytic ( R. mucilaginosa ) and non-uricolytic/ureolytic ( M. asiatica ) yeast species. To confirm the involvement of the yeast uric acid recycling pathway in larval development, we inhibited it by targeting urease, the only enzyme in the yeast recycling pathway absent from the Asian tiger mosquito genome. The impact of this inhibition on the development time of gnotobiotic mosquitoes ( n = 60 per infection status) was assessed using an inhibitor concentration of 2.5 mM. Prior to this experiment, we confirmed that this inhibitor concentration effectively inhibited R. mucilaginosa urease activity without affecting yeast growth or axenic larval development. Statistically significant differences between groups were identified with the Tukey post hoc tests. Columns labelled with an asterisk (*) are significantly different with a p -value < 0.05. ns, not significant

    Article Snippet: An analysis of all publicly available, assembled, and annotated R. mucilaginosa genomes (strains ATCC 58901 and J31 from the JGI MycoCosm portal, as well as the reference strain GDMCC2.30 from NCBI GenBank, accession number PRJNA1034680) confirmed that R. mucilaginosa possess the complete set of genes encoding the uricolytic/ureolytic enzymes required for amino acids synthesis through nitrogen waste recycling.

    Techniques: Inhibition, Infection, Concentration Assay, Activity Assay

    Impact of yeast species and their cell concentrations on oviposition behavior of Ae. albopictus gravid females. The impact of six yeast species ( R. mucilaginosa , A. pullulans , H. uvarum , M. pulcherrima , T. delbrueckii , and M. asiatica ) at two cell concentrations (3.10 2 and 3.10 5 cells.mL −1 ) on the oviposition behavior of gravid females was evaluated using two distinct and complementary laboratory bioassays. Oviposition preference bioassays (corresponding to A & B ) consisted of small-cage three-choice oviposition tests, in which each single gravid female ( n = 24) was allowed to choose between three oviposition sites (double-beaker systems) providing oviposition substrates containing waterborne substances (sterile water or sterile water inoculated with yeasts) and olfactory stimuli (volatile organic compounds emitted by sterile CYM medium or yeast cultures). A Total number of eggs laid by each female per cage, regardless of the selected oviposition site. The pie charts represent the percentages of females that have laid eggs (shown in gray) or have not laid eggs (shown in black). B Number of eggs laid by each female in each oviposition site during three-choice oviposition tests. These oviposition preference bioassays were used to assess short-range attractiveness or repellency of volatile compounds emitted by yeasts but only because they were systematically paired with oviposition stimulation bioassays conducted in 50-mL conical tubes ( C ), where a single gravid female ( n = 25) was specifically exposed to either sterile water or water inoculated with one of the two tested yeast concentrations to enable the evaluation of stimulant or deterrent effects of yeast-derived waterborne substances. C Number of eggs laid by each female according to each type of oviposition substrate. Statistically significant differences between groups were identified with the Tukey post hoc tests. Columns labelled with different letters or with an asterisk (*) are significantly different with a p -value < 0.05. Columns labelled with a dot (•) are almost significantly different with a p -value < 0.1

    Journal: Microbiome

    Article Title: Environmental yeasts differentially impact the development and oviposition behavior of the Asian tiger mosquito Aedes albopictus

    doi: 10.1186/s40168-025-02099-6

    Figure Lengend Snippet: Impact of yeast species and their cell concentrations on oviposition behavior of Ae. albopictus gravid females. The impact of six yeast species ( R. mucilaginosa , A. pullulans , H. uvarum , M. pulcherrima , T. delbrueckii , and M. asiatica ) at two cell concentrations (3.10 2 and 3.10 5 cells.mL −1 ) on the oviposition behavior of gravid females was evaluated using two distinct and complementary laboratory bioassays. Oviposition preference bioassays (corresponding to A & B ) consisted of small-cage three-choice oviposition tests, in which each single gravid female ( n = 24) was allowed to choose between three oviposition sites (double-beaker systems) providing oviposition substrates containing waterborne substances (sterile water or sterile water inoculated with yeasts) and olfactory stimuli (volatile organic compounds emitted by sterile CYM medium or yeast cultures). A Total number of eggs laid by each female per cage, regardless of the selected oviposition site. The pie charts represent the percentages of females that have laid eggs (shown in gray) or have not laid eggs (shown in black). B Number of eggs laid by each female in each oviposition site during three-choice oviposition tests. These oviposition preference bioassays were used to assess short-range attractiveness or repellency of volatile compounds emitted by yeasts but only because they were systematically paired with oviposition stimulation bioassays conducted in 50-mL conical tubes ( C ), where a single gravid female ( n = 25) was specifically exposed to either sterile water or water inoculated with one of the two tested yeast concentrations to enable the evaluation of stimulant or deterrent effects of yeast-derived waterborne substances. C Number of eggs laid by each female according to each type of oviposition substrate. Statistically significant differences between groups were identified with the Tukey post hoc tests. Columns labelled with different letters or with an asterisk (*) are significantly different with a p -value < 0.05. Columns labelled with a dot (•) are almost significantly different with a p -value < 0.1

    Article Snippet: An analysis of all publicly available, assembled, and annotated R. mucilaginosa genomes (strains ATCC 58901 and J31 from the JGI MycoCosm portal, as well as the reference strain GDMCC2.30 from NCBI GenBank, accession number PRJNA1034680) confirmed that R. mucilaginosa possess the complete set of genes encoding the uricolytic/ureolytic enzymes required for amino acids synthesis through nitrogen waste recycling.

    Techniques: Sterility, Derivative Assay

    Volatile organic compounds emitted by yeast species significantly affecting larval development and oviposition behavior of gravid mosquito females. Each yeast culture was prepared in CYM medium at a final volume of 100 mL and an initial cell concentration of 3.10 5 cells.mL. −1 (i.e., concentration shown to significantly impact both larval development and oviposition behavior). Cultures were incubated under constant agitation (130 rpm) for 18 h prior VOC analysis. A Heatmap illustrating the relative abundance of emitted VOCs detected by HS-SPME/GC–MS from five independent replicates of each yeast culture. B , C Relative abundances of 3-methyl- 1-butanol and 2-methyl- 1-butanol, two microbial VOCs shared by both attractive ( R. mucilaginosa and A. pullulans ) and repellent ( M. asiatica and T. delbrueckii) yeast species. The median is indicated by a horizontal black line. Statistically significant differences between yeasts, labelled with different letters, were determined using Dunn’s test with Bonferroni-Holm correction ( p -value < 0.05)

    Journal: Microbiome

    Article Title: Environmental yeasts differentially impact the development and oviposition behavior of the Asian tiger mosquito Aedes albopictus

    doi: 10.1186/s40168-025-02099-6

    Figure Lengend Snippet: Volatile organic compounds emitted by yeast species significantly affecting larval development and oviposition behavior of gravid mosquito females. Each yeast culture was prepared in CYM medium at a final volume of 100 mL and an initial cell concentration of 3.10 5 cells.mL. −1 (i.e., concentration shown to significantly impact both larval development and oviposition behavior). Cultures were incubated under constant agitation (130 rpm) for 18 h prior VOC analysis. A Heatmap illustrating the relative abundance of emitted VOCs detected by HS-SPME/GC–MS from five independent replicates of each yeast culture. B , C Relative abundances of 3-methyl- 1-butanol and 2-methyl- 1-butanol, two microbial VOCs shared by both attractive ( R. mucilaginosa and A. pullulans ) and repellent ( M. asiatica and T. delbrueckii) yeast species. The median is indicated by a horizontal black line. Statistically significant differences between yeasts, labelled with different letters, were determined using Dunn’s test with Bonferroni-Holm correction ( p -value < 0.05)

    Article Snippet: An analysis of all publicly available, assembled, and annotated R. mucilaginosa genomes (strains ATCC 58901 and J31 from the JGI MycoCosm portal, as well as the reference strain GDMCC2.30 from NCBI GenBank, accession number PRJNA1034680) confirmed that R. mucilaginosa possess the complete set of genes encoding the uricolytic/ureolytic enzymes required for amino acids synthesis through nitrogen waste recycling.

    Techniques: Concentration Assay, Incubation, Gas Chromatography-Mass Spectrometry

    Fig. 1 | Early-life fungal colonization and diet-induced obesity model. A Gnotobiotic colonization and diet-induced obesity model. Germ-free dams were gavaged twice with the Oligo-MM12 consortium alone (B; gray) or in combination with C. albicans (B + C; orange), R. mucilaginosa (B + R; pink) or M. restricta (B + M; purple; Males—SD: nB = 23, nB+C = 8, nB+R = 10, nB+M = 12; Females—SD: nB = 18, nB+C = 8, nB+R = 8, nB+M = 12; Males—HFHS: nB = 22, nB+C = 10, nB+R = 8, nB+M = 12; Females—HFHS: nB = 20, nB+C = 12, nB+R = 7, nB+M = 10). Twice in the first week of life, offspring (F1) were exposed to the fungal species corresponding to their coloniza- tion group. F1 were weaned onto standard diet (SD) or high-fat-high-sucrose diet

    Journal: Nature communications

    Article Title: Early-life gut mycobiome core species modulate metabolic health in mice.

    doi: 10.1038/s41467-025-56743-8

    Figure Lengend Snippet: Fig. 1 | Early-life fungal colonization and diet-induced obesity model. A Gnotobiotic colonization and diet-induced obesity model. Germ-free dams were gavaged twice with the Oligo-MM12 consortium alone (B; gray) or in combination with C. albicans (B + C; orange), R. mucilaginosa (B + R; pink) or M. restricta (B + M; purple; Males—SD: nB = 23, nB+C = 8, nB+R = 10, nB+M = 12; Females—SD: nB = 18, nB+C = 8, nB+R = 8, nB+M = 12; Males—HFHS: nB = 22, nB+C = 10, nB+R = 8, nB+M = 12; Females—HFHS: nB = 20, nB+C = 12, nB+R = 7, nB+M = 10). Twice in the first week of life, offspring (F1) were exposed to the fungal species corresponding to their coloniza- tion group. F1 were weaned onto standard diet (SD) or high-fat-high-sucrose diet

    Article Snippet: These consortia included (1) the Oligo-MM1233 alone “B” (A. muris KB18, A. muciniphila YL44, B. caecimuris I48, B. animalis YL2, B. pseudococcoides YL58, C. innocuum I46, E. clostridioforme YL32, E. faecalis KB1, F. plautii YL31, L. reuteri I49, M. intestinale YL27, and T. muris YL45) or (2) in combination with C. albicans (This strain was isolated from infant stool samples enrolled in a clinical study at the Alberta Children’s Hospital in Calgary, Canada80) “B +C”, (3) R. mucilaginosa (DSMZ; DSM 70825) “B +R”, or (4) M. restricta (ATCC;MYA4611) “B +M”.

    Techniques: